Seqtools: Difference between revisions

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[https://linus.nci.nih.gov/seqtools/ BRB-SeqTools]
Wiki for [https://linus.nci.nih.gov/seqtools/ BRB-SeqTools]


= [https://msdn.microsoft.com/en-us/commandline/wsl/about Windows 10 Bash shell] =
= [https://msdn.microsoft.com/en-us/commandline/wsl/about Windows 10 Bash shell] =

Revision as of 12:36, 27 November 2016

Wiki for BRB-SeqTools

Windows 10 Bash shell

Gene counting and variant call (both Samtools and GATK) works fine. For variant annotation, see the comment #2 below.

  1. Need to install Xming. Before calling ./SeqTools from the Bash shell, run export DISPLAY=:0 first.
  2. Automatic setup:
    • Need to install unzip utility sudo apt-get install unzip.
    • Java JDK from ppa:webup8team does not work. We need to install it from Oracle website. To make the installation silently, we need to change the bash script sudo tar xzvf /mnt/c/Users/brb/Downloads/jdk-8u112-linux-x64.tar.gz. Also edit ~/.bashrc file to make the java available from a global environment.
    • An issue in pandoc: timer-create function not implemented. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created.

Software List

See Tools Manager -> Automatic setup. A developer version of the shell script is available on Github.

Program Major language Version
bowtie2 C++ 2.2.6
tophat C++ 2.1.0
bwa C 0.7.12
star C++ 2.5.1b
picard Java 1.141
samtools C 1.3
gatk Java 3.6
bcftools C 1.3
htslib C 1.3
annovar Perl 2016Feb01
sratoolkit Shell 2.7.0
fastqc Java 0.11.5
fastx C 0.0.13
snpeff Java 4_2
htseq Python 0.6.1p1
R R 3.3.x
pandoc Haskell 1.16.0.2
latex, lftp, avfs Ubuntu repository