Seqtools: Difference between revisions

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#* Install fontconfig library '''sudo apt-get install libfontconfig1-dev'''.
#* Install fontconfig library '''sudo apt-get install libfontconfig1-dev'''.
#* New gnome-terminal windows (use apt-get install) cannot be opened from Bash shell. This affect Automatic setup tools in Tools manager and profile manager.
#* New gnome-terminal windows (use apt-get install) cannot be opened from Bash shell. This affect Automatic setup tools in Tools manager and profile manager.
#* Java JDK from ppa:webup8team does not work. We need to install it from Oracle website. To make the installation silently, we need to change the bash script '''sudo tar xzvf /mnt/c/Users/brb/Downloads/jdk-8u112-linux-x64.tar.gz'''. Also edit '''~/.bashrc''' file to make the java available from a global environment.
#* Java JDK from ppa:webup8team does not work. We need to download/install it <jdk-8u112-linux-x64.tar.gz> from Oracle website. To make the installation silently, we need to add two lines to the installation script. See [https://javaguru.fi/unattended-java-install-ubuntu-14-04.html here] for apt-get approach and [https://www.digitalocean.com/community/tutorials/how-to-manually-install-oracle-java-on-a-debian-or-ubuntu-vps here] for tarball approach.  
#* An issue in pandoc: [https://groups.google.com/forum/#!msg/pandoc-discuss/BI51p5YKpa0/mO5xowuZAwAJ timer-create function not implemented]. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created.
#* An issue in pandoc: [https://groups.google.com/forum/#!msg/pandoc-discuss/BI51p5YKpa0/mO5xowuZAwAJ timer-create function not implemented]. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created.



Revision as of 16:06, 28 November 2016

Wiki for BRB-SeqTools

Windows 10 Bash shell

Gene counting and variant call (both Samtools and GATK) works fine. For variant annotation, see the comment #2 below.

  1. Need to install Xming. Before calling ./SeqTools from the Bash shell, run export DISPLAY=:0 first.
  2. Automatic setup:
    • Need to install unzip utility sudo apt-get install unzip.
    • Install fontconfig library sudo apt-get install libfontconfig1-dev.
    • New gnome-terminal windows (use apt-get install) cannot be opened from Bash shell. This affect Automatic setup tools in Tools manager and profile manager.
    • Java JDK from ppa:webup8team does not work. We need to download/install it <jdk-8u112-linux-x64.tar.gz> from Oracle website. To make the installation silently, we need to add two lines to the installation script. See here for apt-get approach and here for tarball approach.
    • An issue in pandoc: timer-create function not implemented. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created.

Software List

See Tools Manager -> Automatic setup. A developer version of the shell script is available on Github.

Program Major language Version
bowtie2 C++ 2.2.6
tophat C++ 2.1.0
bwa C 0.7.12
star C++ 2.5.1b
picard Java 1.141
samtools C 1.3
gatk Java 3.6
bcftools C 1.3
htslib C 1.3
annovar Perl 2016Feb01
sratoolkit Shell 2.7.0
fastqc Java 0.11.5
fastx C 0.0.13
snpeff Java 4_2
htseq Python 0.6.1p1
R R 3.3.x
pandoc Haskell 1.16.0.2
latex, lftp, avfs Ubuntu repository