Seqtools: Difference between revisions
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#* Install fontconfig library '''sudo apt-get install libfontconfig1-dev'''. | #* Install fontconfig library '''sudo apt-get install libfontconfig1-dev'''. | ||
#* New gnome-terminal windows (use apt-get install) cannot be opened from Bash shell. This affect Automatic setup tools in Tools manager and profile manager. | #* New gnome-terminal windows (use apt-get install) cannot be opened from Bash shell. This affect Automatic setup tools in Tools manager and profile manager. | ||
#* Java JDK from ppa:webup8team does not work. We need to install it from Oracle website. To make the installation silently, we need to | #* Java JDK from ppa:webup8team does not work. We need to download/install it <jdk-8u112-linux-x64.tar.gz> from Oracle website. To make the installation silently, we need to add two lines to the installation script. See [https://javaguru.fi/unattended-java-install-ubuntu-14-04.html here] for apt-get approach and [https://www.digitalocean.com/community/tutorials/how-to-manually-install-oracle-java-on-a-debian-or-ubuntu-vps here] for tarball approach. | ||
#* An issue in pandoc: [https://groups.google.com/forum/#!msg/pandoc-discuss/BI51p5YKpa0/mO5xowuZAwAJ timer-create function not implemented]. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created. | #* An issue in pandoc: [https://groups.google.com/forum/#!msg/pandoc-discuss/BI51p5YKpa0/mO5xowuZAwAJ timer-create function not implemented]. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created. | ||
Revision as of 16:06, 28 November 2016
Wiki for BRB-SeqTools
Windows 10 Bash shell
Gene counting and variant call (both Samtools and GATK) works fine. For variant annotation, see the comment #2 below.
- Need to install Xming. Before calling ./SeqTools from the Bash shell, run export DISPLAY=:0 first.
- Automatic setup:
- Need to install unzip utility sudo apt-get install unzip.
- Install fontconfig library sudo apt-get install libfontconfig1-dev.
- New gnome-terminal windows (use apt-get install) cannot be opened from Bash shell. This affect Automatic setup tools in Tools manager and profile manager.
- Java JDK from ppa:webup8team does not work. We need to download/install it <jdk-8u112-linux-x64.tar.gz> from Oracle website. To make the installation silently, we need to add two lines to the installation script. See here for apt-get approach and here for tarball approach.
- An issue in pandoc: timer-create function not implemented. In other words, if we run variant annotation, we will get a bug report message. The main output files (1 vcf and 2 texts files) are generated but the pdf/html files cannot be created.
Software List
See Tools Manager -> Automatic setup. A developer version of the shell script is available on Github.
Program | Major language | Version |
---|---|---|
bowtie2 | C++ | 2.2.6 |
tophat | C++ | 2.1.0 |
bwa | C | 0.7.12 |
star | C++ | 2.5.1b |
picard | Java | 1.141 |
samtools | C | 1.3 |
gatk | Java | 3.6 |
bcftools | C | 1.3 |
htslib | C | 1.3 |
annovar | Perl | 2016Feb01 |
sratoolkit | Shell | 2.7.0 |
fastqc | Java | 0.11.5 |
fastx | C | 0.0.13 |
snpeff | Java | 4_2 |
htseq | Python | 0.6.1p1 |
R | R | 3.3.x |
pandoc | Haskell | 1.16.0.2 |
latex, lftp, avfs | Ubuntu repository |